Multiwell-MEA-System

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Overview

The Multiwell-MEA system is the perfect solution for medium to high throughput in vitro electrophysiology. Featuring a 24- or 96-well plate format, it is especially suited for safety pharmacology and toxicity screenings.

Being based on the MEA2100-technology, it includes high-quality, low-noise amplifiers, and freely-programmable stimulators.

One big advantage of the Multiwell-MEA-System is the high sampling rate. Your data is sampled at up to 50 kHz per channel (simultaneously on all channels). Thus, the accuracy of your data is guaranteed, whether you record from cardiac or neuronal samples.

The data is recorded and analyzed by the included software Multiwell-Screen.

The MEA Xpress can integrate the Multiwell-MEA-System and offers automated liquid handling.

For more detailed information, please click on the description tab above.

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IFB-C

As part of the system, the IFB-C multiboot is a new interface board generation which is able to receive data from a MEA2100(-Mini)-Headstage, a Multiwell-MEA-Headstage, a CMOS-MEA5000-Headstage, a MEA2100-Beta-Screen-Headstage, a ME2100-Headstage, or a W2100-Receiver. This makes cost-effective combinations with only one interface board and multiple recording systems possible. You can establish for example your experiments on 60, 120, or 256 electrodes with the MEA2100-System, then add the Multiwell-MEA-Headstage and start high-throughput screening. It's also possible to use the same interface board for in vitro MEA experiments and tethered / wireless in vivo recordings. This leaves you with even more flexibility in switching between possible configurations for your specific research needs.

Key features:

Real-time signal detection and feedback*
Freely programmable DSP*
Multiple inputs/outputs, including digital, analog, and audio
SuperSpeed USB 3.0 ports

* applicable for MEA2100(-Mini), ME2100, and W2100

Read more technical specifications

Description

The new Multiwell-MEA-System from Multi Channel Systems

The Multiwell-MEA-System consists of 4 components: Well plates, headstage, interface board, and PC with software. Please click on the arrows on the right to learn more about the single components.

Well plates: Transparent base, high quality electrodes

Multi Channel Systems offers various kinds of SBS-compliant well plates.

The 24-well plate has 12 electrodes per well, arranged in a 4x4 grid.
The 96-well plate has 3 or 12 electrodes per well.

In all well plates, there is an internal reference electrode in each well and each electrode can be selected for stimulation.

The 24-well plate features opaque or transparent glass base material, so you can visually check your cells under a microscope. The electrodes can be as small as 30 µm for neuronal cells or 100 µm for cardiac samples. The electrodes are either of gold (100 µm) or – like all our standard MEA-electrodes – of Titanium Nitride (30 µm). You can go into higher throughput with the same electrode quality as with the low-impedance, high signal-to-noise ratio single-well MEAs.

The 96-well plates are the perfect solution for high throughput screening. The electrodes are made of gold and make those well plate an economical solution for screening experiments.

On the bottom of the well plates, you will see the contact pads, which connect to the contact pins of the headstage.

Headstage: Powerful amplifier, high sampling rate, integrated stimulation and heating

The headstage is the core element of the system. It houses the well plate, amplifies and digitizes the signals and has an integrated stimulus generator.

The brackets can be opened and closed either by pushing the buttons on the housing or with a single mouse click in the software. By closing the brackets, the contact pads of the well plate connect to the contact pins.

Featuring 288 channels, the built-in amplifier makes sure that the recorded signals are amplified close to the signal source, thereby minimizing noise. The data is then sampled at 50 kHz/channel on all channels simultaneously, ensuring an excellent data quality.

The headstage also provides an integrated stimulator for current and voltage driven stimulation. You can select any electrode for stimulation and design the signal shape via the included software Multiwell-Screen.

For long-term recordings under stable conditions, the recording chamber can be covered with a lid, which has an inlet for CO2. The complete headstage can be placed in a dry incubator, if needed. Additionally, it has an integrated temperature controller which regulates the temperature of the heating plate right below the well plate.

Interface board: Synchronization with other instruments

The interface board receives data from the headstage via iX industrial type B cable. In the interface board you find various analog and digital in- and outputs for synchronization with other instruments. The interface board connects to the computer via USB 3.0 SuperSpeed. The IFB-C is a new interface board generation which is able to receive data from MEA2100-Headstages, Multiwell-MEA-Headstage, CMOS-MEA5000-Headstage, ME2100-Systems or W2100-Systems. This makes cost-effective combinations with only one interface board and multiple recording systems possible.

IFB-C

Multiwell-Screen Software: Easy to use, specialized for screenings

The last component of the system is the computer with the included software package Multiwell-Screen. The software is designed specifically for the needs of screening experiments with the Multiwell-MEA-System. With only a few mouse clicks, you can set-up your experiment, including information on applied drugs and wash-in phases. The software then guides you through your experiment and calculates dose-response curves automatically online, while you can select between different parameters (field potential duration, peak-to-peak amplitude, RR interval, spike count etc). You can display your data in real-time and filter it (high-pass, low-pass, Butterworth, Notch etc.). Moreover, you can detect spikes and also record digital events.

Within the same software, you can design the stimulation patterns and decide when you want to stimulate.

The separate analysis software (Multiwell Analyzer), which is also included in the system, provides further tools for detailed offline analysis.

Both tools are designed for repeated screening experiments, so saving your settings and experimental layout is quick and easy.

As a policy at Multi Channel Systems, updates of the Multiwell Screen software package will be available online and free of charge, so you are always up-to-date with recent developments.

Liquid Handling: Flexible and precise dilution and dispension

MCS combined the reliable and cost efficient Opentrons OT-2 pipetting robot with the well known Multiwell-MEA-System to generate an automated platform for substance screening: MEA Xpress. One software is used to automatically generate a compound plate from medium and compound stock with the pipetting robot, control the electrophysiological recording, and apply the drugs from the compound plate to the cells during the experiment without further user interference.
The robot offers high-precision pipettes that execute the liquid transfer quickly and accurately. Being compact, it fits on your bench with ease.
The robot perfectly houses the Multiwell-MEA-System. However, you can always remove the recording system for benchtop operation.

Support and Sales team: Always there for you

Multi Channel Systems has a team of highly-qualified scientists, whose only task is to help you with any issue you might have. No matter whether you want to know whether your biological sample is suitable for MEA recordings or if you have a specific software question: Our support team is here to help you.

We have scientists who earned their PhDs in the fields of biology, biophysics, and biochemistry and specialized in:

Neuronal electrophysiology
Cardiac electrophysiology
Xenopus oocyte research
Patch Clamp

Our sales team has years of experience in electrophysiology, too. They can help you in choosing the right system for your specific application, give you advice on the general set-up and useful accessories. They are happy to visit you in your lab anytime to:

Make a demonstration of our systems
Help you with the installation of a new system
Give you training on hard- and software
Discuss any issues you might have with your application

Worldwide, we are supported by our distribution partners. We have long-lasting cooperations with all of our partners and they are regularly trained on our equipment. Be sure that they have the expertise to advise you on all MCS-Systems.

Please do not hesitate to contact us. Normally, we reply to you within 24 hours. You can contact us via:

Phone +49 7121 90925 25
Email support [at] multichannelsystems.com (Support Team); sales [at] multichannelsystems.com (Sales Team)
Contact form

Benefits

The Multiwell-MEA-System from Multi Channel Systems offers the following benefits:

Sampling rate of 50 kHz per channel ensures accurate data output.
High-quality well plates with 12 or 3 electrodes per well. One internal reference electrode per well. Each electrode is selectable for stimulation.
Standard well plate format facilitates exchange between 24- or 96-well plates.
Integrated stimulator (voltage driven stimulation), no other device necessary and the complete system is software controlled.
Multiwell-Screen software specialized on screening experiments is included (updates available for free).
Integrated heating as well as a connection to CO₂ supply enables full climate control of the recording chamber.

Media

Videos:

Video tutorials:

Multiwell-Screen - Experiment Setup

Products

Documents

Manual:

Multiwell-MEA-System_Manual.pdf

Data sheet:

MCS_Multiwell-MEA-System_Datasheet.pdf

Brochure:

Cardiac-Applications_Brochure.pdf

Neuro-Applications-InVitro_Brochure.pdf

MCS_MEA_Xpress_Multiwell-brochure.pdf

Customer voices:

MCS_Customer-Voices_Multiwell-MEA_Samie-Patel.pdf

MEAs

Multiwell MEA Plates

Multiwell plates for the Multiwell-MEA-System:

24 or 96 wells, 288 recording electrodes, 12 or 3 electrodes with reference per well, FR4 or transparent glass substrate, Sterilized by gamma radiation. Not for medical use.

Accessories

Tabs

Products

Publications

Please note: Currently, our publications library includes articles up to 2021 only. Contact us for publications from 2022 – present. Contact Us

Frega M, Linda K, Keller JM, Gumus-Akay G, Mossink B, van Rhijn JR, Negwer M, Klein Gunnewiek T, Foreman K, Kompier N, Schoenmaker C, van den Akker W, van der Werf I, Oudakker A, Zhou H, Kleefstra T, Schubert D, van Bokhoven H, Nadif Kasri N (2019) Neuronal network dysfunction in a model for Kleefstra syndrome mediated by enhanced NMDAR signaling. Nat Commun 10:4928.

Frega M, Linda K, Keller JM, Gümüş-Akay G, Mossink B, van Rhijn J-R, Negwer M, Gunnewiek TK, Foreman K, Kompier N, Schoenmaker C, van den Akker W, Oudakker A, Zhou H, Kleefstra T, Schubert D, van Bokhoven H, Kasri NN (2019) Neuronal network dysfunction in a human model for Kleefstra syndrome mediated by enhanced NMDAR signaling. bioRxiv:585596.

Jackson MR, Loring KE, Homan CC, Thai MH, Maattanen L, Arvio M, Jarvela I, Shaw M, Gardner A, Gecz J, Shoubridge C (2019) Heterozygous loss of function of IQSEC2/Iqsec2 leads to increased activated Arf6 and severe neurocognitive seizure phenotype in females. Life Sci Alliance 2.

Kizner V, Fischer S, Naujock M (2019) Multielectrode Array (MEA)-Based Detection of Spontaneous Network Activity in Human iPSC-Derived Cortical Neurons. Methods Mol Biol 1994:209-216.

Kizner V, Naujock M, Fischer S, Jager S, Reich S, Schlotthauer I, Zuckschwerdt K, Geiger T, Hildebrandt T, Lawless N, Macartney T, Dorner-Ciossek C, Gillardon F (2019) CRISPR/Cas9-mediated Knockout of the Neuropsychiatric Risk Gene KCTD13 Causes Developmental Deficits in Human Cortical Neurons Derived from Induced Pluripotent Stem Cells. Mol Neurobiol.

Klein Gunnewiek TM, Van Hugte EJH, Frega M, Guardia GS, Foreman KB, Panneman D, Mossink B, Linda K, Keller JM, Schubert D, Cassiman D, Morava E, Rodenburg R, Perales-Clemente E, Nelson TJ, Kasri NN, Kozicz T (2019) Mitochondrial dysfunction impairs human neuronal development and reduces neuronal network activity and synchronicity. bioRxiv:720227.

Kraushaar U, Guenther E (2019) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. Methods Mol Biol 1994:197-208.

Mendis GDC, Berecki G, Morrisroe E, Pachernegg S, Li M, Varney M, Osborne PB, Reid CA, Halgamuge S, Petrou S (2019) Discovering the pharmacodynamics of conolidine and cannabidiol using a cultured neuronal network based workflow. Sci Rep 9:121.

Morrisroe E (2019) Multielectrode Arrays: A tool for modellig genetic epilepsy. FACULTY OF MEDICINE, DENTISTRY & HEALTH SCIENCES

Patel AM, Wierda K, Thorrez L, van Putten M, De Smedt J, Ribeiro L, Tricot T, Gajjar M, Duelen R, Van Damme P, De Waele L, Goemans N, Tanganyika-de Winter C, Costamagna D, Aartsma-Rus A, van Duyvenvoorde H, Sampaolesi M, Buyse GM, Verfaillie CM (2019) Dystrophin deficiency leads to dysfunctional glutamate clearance in iPSC derived astrocytes. Transl Psychiatry 9:200.

Sfakianou A (2019) Functional Characterization of Human iPSC-Derived Neural Networks using MEA Systems for in vitro Modeling of Psychiatric Disorders.

van Meer BJ, Krotenberg A, Sala L, Davis RP, Eschenhagen T, Denning C, Tertoolen LGJ, Mummery CL (2019) Simultaneous measurement of excitation-contraction coupling parameters identifies mechanisms underlying contractile responses of hiPSC-derived cardiomyocytes. Nat Commun 10:4325.

Edwards SL, Zlochiver V, Conrad DB, Vaidyanathan R, Valiquette AM, Joshi-Mukherjee R (2018) A Multiwell Cardiac muGMEA Platform for Action Potential Recordings from Human iPSC-Derived Cardiomyocyte Constructs. Stem Cell Reports.

Mulder P, de Korte T, Dragicevic E, Kraushaar U, Printemps R, Vlaming MLH, Braam SR, Valentin JP (2018) Predicting cardiac safety using human induced pluripotent stem cell-derived cardiomyocytes combined with multi-electrode array (MEA) technology: A conference report. J Pharmacol Toxicol Methods 91:36-42.

Sun Y, Gao Y, Tidei JJ, Shen M, Hoang JT, Wagner D, Zhao X (2018) Loss of MeCP2 in immature neurons leads to impaired network integration. Hum Mol Genet.

Telezhkin V, Straccia M, Yarova P, Pardo M, Yung S, Vinh NN, Hancock JM, Barriga GG, Brown DA, Rosser AE, Brown JT, Canals JM, Randall AD, Allen ND, Kemp PJ (2018) Kv7 channels are upregulated during striatal neuron development and promote maturation of human iPSC-derived neurons. Pflugers Arch.

Gazina EV, Morrisroe E, Mendis GDC, Michalska AE, Chen J, Nefzger CM, Rollo BN, Reid CA, Pera MF, Petrou S (2017) Method of derivation and differentiation of mouse embryonic stem cells generating synchronous neuronal networks. J Neurosci Methods.

Mendis GDC (2017) Detection of Phenotypic Network Signatures in Cultured Neuronal Networks. Department of Mechanical Engineering.

Shen N, Knopf A, Westendorf C, Kraushaar U, Riedl J, Bauer H, Pöschel S, Layland SL, Holeiter M, Knolle S, Brauchle E, Nsair A, Hinderer S, Schenke-Layland K (2017) Steps toward Maturation of Embryonic Stem Cell-Derived Cardiomyocytes by Defined Physical Signals. Stem Cell Reports 9:122-135.

Rast G, Kraushaar U, Buckenmaier S, Ittrich C, Guth BD (2016) Influence of field potential duration on spontaneous beating rate of human induced pluripotent stem cell-derived cardiomyocytes: Implications for data analysis and test system selection. Journal of Pharmacological and Toxicological Methods 82:74-82.

Sala L, Yu Z, Ward-van Oostwaard D, van Veldhoven JP, Moretti A, Laugwitz KL, Mummery CL, AP IJ, Bellin M (2016) A new hERG allosteric modulator rescues genetic and drug-induced long-QT syndrome phenotypes in cardiomyocytes from isogenic pairs of patient induced pluripotent stem cells. EMBO Mol Med 8:1065-1081.

www.multichannelsystems.com

Innovations in Electrophysiology

Multiwell-MEA-System

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IFB-C

Key features: