Multiwell-Screen

Software Version:

V 2.0.11.0

Release Date:

2023-08-23

Software Download:

Multiwell-Screen Setup

With the Multiwell-Screen software you can run and control experiments with Multiwell-MEA-Systems.

Features are:

Supports 24- and 96-well plates
Simultaneous measurement of dose-dependent effects of different compounds on cardiomyocytes and neurons
Analysis of measured signals during the recording:
Cardiac or neuronal application:
- Beat detection
- Spatial distribution of beat latency
- Beat intervals
- Averages
- Detection of field potential duration
- Spike detection
- Spike counts
- Neuro: Network burst detection
Computation of Dose-Response curves
PDF report generator
Export displayed data as images, .xlsx, and .csv files
Replay recorded files to optimize the analysis

Compatible with Windows^® 8.1 / 10 (64 Bit).

Product Category:

Products

In Vitro MEA-Systems

Software

In vitro / in vivo Systems

Multiwell-Screen

References

Release Notes

Version 2.0.11.0

Added MEA Xpress lab ware definition for Axygen 96 Deep Well Plate 2.0 ml
Set Axygen 96 Deep Well Plate 2.0 ml as default mixing plate in MEA Xpress Wizard
Deactivated Hdf5 raw data recording in default settings

Version 2.0.10.1

improved pipette/labware calibration GUI in MEA Xpress Wizard
bugfix: reliable dilution mixing after each transfer (MEA Xpress preparation script)
bugfix: no wasting of unused pipette tips (MEA Xpress preparation script)
bugfix: proceed after “Script” step in MEA Xpress Wizard (advanced mode)

Version 2.0.9.1

New Feature: HDF5 recording of raw data and spikes (optional, default: on)
Bugfix: well selection via single click in multiwell selection panel (main window, stimulator settings, compound/well assignment)
Bugfix: MEA Xpress door state check during labware calibration
Bugfix: initial plate selection when opening the “Override Plate Type” dialog

Version 2.0.8.0

New Feature: Option for mandatory Plate ID in the Lab Book.
Bugfix: new lab ware arrangement (MEA Xpress).

Version 2.0.2.0

New Feature: Support for Multiwell Headstages connected to port 2/B.
Update: FW updates for USB, FPGA IF, HS.
Update: Corrected plate movement distance if pipetting lid is used with MEA Xpress.
Update: Disabled Headstage Touch Pads in MEA Xpress mode.
Bugfix: Fixed loading of settings in MEA Xpress mode.

Version 2.0.1.0

Update: Added „Load Recordings“ button to the Application menu of the Replayer.
Update: Added „Delete Default Configuration“ button to the Application menu.
Bugfix: Better handling of loading default configuration files created with a different experiment definition
Bugfix: Better handling of loading configuration files created with a different experiment definition: Loading of the experiment definition is now optional.
Bugfix: Fixed freeze of MW-Opto-Stim with continuously lit LEDs if Pause of 0 ms was used
Bugfix: in Simple Recording mode, wells assigned to compounds were sometimes deleted in the Compound assignment dialog
Bugfix: Fixed driver installation

Version 2.0.0.0

This is a major update for Multiwell-Screen. It features a reworked, more modular UI that can be customized to the needs of your experiment, support for the MEA Xpress system, the ability to divide recordings into multiple files, parallel use of multiple Multiwell headstages and much more. See below for a list of the most important changes and new features:

New Feature: New Software Startup Dialog
New Feature: Support for MEA Xpress (Standalone and with Headstage)
New Feature: New View Recordings Dialog to open files in Replayer and Analyzer
New Feature: UI adapts to selected experiment type
New Feature: UI adapts to selected software modules
New Feature: Support for multi file recordings
New Feature: New UI to apply analysis settings to experiments or well
New Feature: Required hardware devices are checked and monitored in the software
New Feature: Up to 8 parallel Multiwell-Screen instances possible
Update: Reworked standalone LED stimulation
Update: Timing protocols work for standalone LED stimulation
Update: Simplified the "Compound Settings" Dialog (depending on experiment type)
Update: Recording of unassigned wells can be disabled
Bugfix: Fixed data scaling bug with the IFB-C
Bugfix: Set IFB-C IO level properly for MW-OptoStim devices
Many other bugfixes and UI tweaks

Version 1.11.7.0

New Firmware: FPGA-IF 2.06 for IFB-C
Firmware Update: USB 1.74, FPGA IF 0.20
Bugfix: After manually skipping the Recording phase, spike timestamps were sometimes detected for 100 ms after the skip
Bugfix: Neuro: Fixed crash when updating Spike Analyzer settings after stopping stimulation

Version 1.11.6.0

Firmware Update: USB 1.73
Firmware Update: MW24-opto-stim 0.32 and MW96-opto-stim 0.32
Update: Support for new 96 well plate 96W700/100G-288
Update: Performance improvement for compound assignment dialog
Update: Show warning if IFB is connected to a slow USB port
Bugfix: Fixed Coefficient of Variation computation for Dose-Response Curves
Bugfix: Neuro: Fixed spike threshold computation with multiple segments (starting from the 2nd segment, the first 100 ms in each segment had been ignored for this)
Bugfix: Cardio: Fixed heartbeat 2D latency analysis

Version 1.11.5.0

Bugfix: Fixed crash when loading default settings file on startup
Bugfix: Fixed error message when a 2^nd Multiwell-Screen instance tries to connect to the same device

Version 1.11.4.0

New Feature: 24- and 96-well LED Stimulator support
Firmware Update: HS1 0.61
Update: Neuro: Use identical y-axis scaling for PSTHs
Update: Neuro: Added PSTH computation relative to LED stimulation timestamps
Update: Removed possibility to deactivate Digital-In recording, because it interfered with the LED stimulator communication
Bugfix: Loading a settings template should not override the plate layout
Bugfix: Neuro: Fixed spike count computation for network bursts

Version 1.10.3.0

Firmware Update: USB 1.69 for improved plate movement for test dummy plate
Firmware Update: HS1 0.60 to reduce device power consumption and remove temperature issues

Version 1.10.2.0

Firmware Update: USB 1.67 with improved plate movement for certain plates

Version 1.10.1.0

New Feature: Scheduler in the Experimental Flow Dialog for fine-grained experiment timing definition
Update: Added option to the Recorder Settings Dialog to disable raw data recording
Update: Added export to Excel file format for analysis results
Update: Support for new plate types
Update: Support for new Cardio2D Layouts
Several minor bug fixes

Version 1.9.2.0

New Feature: Cardio: Conduction Velocity (dose-response curves, pdf report and ASCII export)
New Feature: ASCII import for stimulation patterns (MC_Stimulus II format)
Update: Multiple stimulation electrodes per well for voltage controlled stimulation
Update: Added "Once" stimulation protocol, where a stimulation pattern is only run once
Bugfix: Dig-In signals on the IFB are properly recorded now
Bugfix: Neuro: A Network Burst is only exported once per well to ASCII, not once per channel
Several UI and stability bug fixes

Version 1.9.1.0

New Feature: Neuro: Network Burst Detection
Firmware Update: USB 1.66 for improved plate movement for certain plates
Update: Improved UI for result export to CSV dialog
Update: Neuro: New Network Burst Dose-Response Parameters in Dose-Response-Display
Update: Neuro: New Display Styles in Multiwell Selection Panel: Bursts and Network Bursts
Update: Neuro: New Network Burst Display Parameters in Result Per Channel Display
Update: Neuro: New Network Burst options for results export to CSV
Update: Neuro: Added Network Burst parameters to PDF report, reorganized well result tables
Update: Cardio: T Detection runs after the end of the Explore phase
Bugfix: Fixed error in compound settings dialog when assigning doses to wells
Bugfix: Fixed crash when modifying spike detection thresholds for deactivated channel
Bugfix: Fixed file name generation if neither Lab Book nor Recorder Settings Dialog has been opened
Bugfix: Neuro: Fixed export to CSV and PNG for PSTH-Display
several minor UI improvements

Version 1.8.1.0

Update: Freely labelled "dilution" series
Update: Start offset for stimulation
Bugfix: Fixed an issue that could lead to a crash at the end of the experiment
Bugfix: Fixed enabling the Temperature Control display for non-integer setpoint temperatures
Several minor bugfixes for increased stability

Version 1.7.4.0

Firmware Update: FPGA IF V. 0.18
Update: Added support for new 96 well Mux plate
Bugfix: Revert mouse pointer to default after dragging of the spike threshold line is finished
Bugfix: Corrected font for plate layout in the experiment settings chapter of the report

Version 1.7.3.0

Update: Added Dilution Series to Compound Database
Bugfix: Fixed crash when icon scaling is set to 125 % on Windows 8.1

Version 1.7.1.0

New feature: Raw Data Overview Display for all channels
New feature: Database for Compound Metadata
Update: Support for new 96 well glass plate type
Update: Added plate name to report
Bugfix: Fixed rare crashes in the last analysis phase
Bugfix: Cardio: Fixed default value for user-defined HB detection threshold
Bugfix: Fixed wrong exponent in ASCII Export from Dose-Response Display

Version 1.6.6.0

Update: Support for new 24 well glass plate
Bugfix: Fixed legends in pdf reports

Version 1.6.5.0

New feature: automatic check for updates
Update: Support for 96 well plate with 1152 channels
Lots of Bugfixes

Version 1.5.9.0

New feature: Overwrite detected plate type via Command -> Override Plate Type
Update: Support for new 24 well glass plate type
Bugfix: Fixed stimulator blanking if Sync Out is turned off

Version 1.5.6.0

New feature: Burst stimulation

Version 1.5.5.0

Update: Show plate layout in status bar
Bugfix: Fixed installation of USB driver
Bugfix: Fixed display of help files when .htm files are not associated with a browser

Version 1.5.4.0

Update: Cardio: Better default parameters for T detection.
Update: Show warning if no channels are selected at experiment start.
Bugfix: Fixed compound selection dialog for font scaling > 100 %
Bugfix: Cardio: User defined threshold was reset by opening/closing Analyzer settings
Bugfix: Neuro: Fixed several special cases for the Burst Detector
Bugfix: Neuro: PSTH - Display should not get updated outside the analysis phase.
Bugfix: Replayer: Fixed possible crash when pressing "Stop Explore"
Bugfix: Replayer: Fixed crash when loading files with non-cumulative dilution series.

Version 1.5.1.0

Bugfix: Compound Settings dialog: Fixed that defining a new compound could override the default dilution series setting of the last compound
Bugfix: Experiment Flow dialog: Fixed rare error message
Bugfix: Neuro: Fixed crash when stimulation is running
Bugfix: Neuro: Fixed rare error message in the Replayer
Bugfix: Neuro: Fixed burst detection for some special cases
Bugfix: Cardio: Fixed reading the Noise Suppression parameter
Firmware update

Version 1.5.0.0

New feature: Non-cumulative dilution series
New feature: Distinct dilution series per compound
New feature: Access to firmware filters
New feature: Definition of an analysis region within the recording
New feature: Neuro: Visualization of single burst parameters per channel
New feature: Neuro: Spike Rate as Dose-Response parameter

Version 1.3.4

Bugfix: Corrected the layout of the 72 well plates

Version 1.3.3

Bugfix: Fixed that the default configuration directory was not created during the installation procedure
Bugfix: Fixed a crash in the Replayer when loading a recording not containing well A1 + a result file created by the Replayer.
Bugfix: Fixed a crash if Multiwell-Screen is started with a very specific window arrangement.
Update: Set the Raw Data Display always as the top-most tab page
Firmware Update: Updated headstage firmware to 0.48

Version 1.3.2

Update: Detection of new test dummy plate
Bugfix: Fixed crash in the Replayer when trying to read data recorded with high pass filter at either 0.1 or 0.5 Hz
Bugfix: Cardio mode: Fixed crash when Averager post interval was set to more than 1 second. New upper limit for the post interval is 3 seconds.

Version 1.3.1

New: Monopolar current stimulation
New: Burst detector (Neuro)
New: Integrated help system
New: Built-in Firmware update
Update: Improved automatic channel selection in Neuro mode
Update: Automatic spike threshold estimation can take the minimum of several time windows
Update: Peristimulus time histogram (PSTH) plot display now working as expected
Update: Temperature controller starts automatically with the software and keep its state on close
Update: User can select the plate type manually (if automatic detection fails)
Several bug fixes and UI improvements
NOTE: Before running Multiwell-Screen Version 1.3.1 for the first time, please download the Multiwell-Update.zip archive, extract it and run Multiwell-Update.exe. Press the available "Update" buttons to update the firmware. After all updates have finished, start Multiwell-Screen version 1.3.1.

Version 1.2.4

Bugfix: Replayer could not handles files with deactivated wells
Added button "Reset Window Arrangement" to revert to default docking state
Several minor bug fixes and UI improvements
Software is now 64-Bit only

Version 1.2.2

Restricted installation to Windows 8.1 and newer
Bugfix for standard deviation computation (neuro mode)
Improved report (cardio mode)
Several bug fixes

Version 1.2.0

Initial release

Documents

Manual:

Multiwell-MEA-System_Manual.pdf

Media

Video tutorials: