CMOS-MEA5000-System

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Overview

The CMOS-MEA5000-System is the in vitro recording system from Multi Channel Systems for extracellular recordings and stimulation at the highest resolution.

Based on the complementary metal-oxide semiconductor technology, it opens up new possibilities in electrophysiological research.

With more than 4000 recording sites, each of them sampled at 25 kHz, the chip allows extracellular recordings at a very high spatio-temporal resolution. By including amplification on the chip itself, noise is minimized and a high signal quality is guaranteed.

For more detailed information, please click on the description tab above.

High resolution: The video shows the propagation of an average action potential along a single unmyelinated axon of a rat ganglion cell. Details can be found in the paper of H.Stutzki et al. in Frontiers Cell. Neurosci. 8:38 (2014).
Data courtesy to F.Jetter, H.Stutzki and G.Zeck, NMI Reutlingen

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IFB-C

As part of the system, the IFB-C multiboot is a new interface board generation which is able to receive data from a MEA2100(-Mini)-Headstage, a Multiwell-MEA-Headstage, a CMOS-MEA5000-Headstage, a MEA2100-Beta-Screen-Headstage, a ME2100-Headstage, or a W2100-Receiver. This makes cost-effective combinations with only one interface board and multiple recording systems possible. You can establish for example your experiments on 60, 120, or 256 electrodes with the MEA2100-System, then add the Multiwell-MEA-Headstage and start high-throughput screening. It's also possible to use the same interface board for in vitro MEA experiments and tethered / wireless in vivo recordings. This leaves you with even more flexibility in switching between possible configurations for your specific research needs.

Key features:

Real-time signal detection and feedback*
Freely programmable DSP*
Multiple inputs/outputs, including digital, analog, and audio
SuperSpeed USB 3.0 ports

* applicable for MEA2100(-Mini), ME2100, and W2100

The chip is based on complementary metal oxide semiconductor (CMOS) technology, facilitating fast, high-resolution imaging of electrical activity.

The chip is equipped with a culture chamber to house your sample, while allowing the use of a microscope.

The recording sites are arranged in a 65x65 grid and have a 16µm or 32µm interelectrode distance (center to center). Between the recording electrodes, there is a grid of 32x32 bigger stimulation sites. Summarizing, you can record from 4225 electrodes and stimulate your sample at 1024 sites.

The high number of electrodes also results in a large recording area (1 mm² @ 16 µm; 4 mm² @ 32 µm). Thereby, you can see the signals from every single cell and even the signal propagation along an axon, while still getting an overview on your complete sample, e.g. a cell culture and see how the cells interact.

The chip is coated with planar oxide, similar to glass, enhancing the biocompatibility and biostability.

Chamber for experiments under dark light conditions

The dark chamber allows to do experiments under stable, dark light conditions, while still providing optional access for reference electrodes and perfusion.

It is included with every CMOS-MEA5000-System and consists of a ring (picture below on the right), that is snapped on the headstage and a lid (picture below on the left), that is then put on the ring for shielding light from your sample. More information can be found here.

CMOS-MEA-Headstage with integrated stimulator

The core of the system is the headstage, housing the chip with your sample. It samples the data coming from the chip at 25 kHz per channel (all electrodes simultaneously), thus no signal is lost – even axonal spikes are displayed and recorded thoroughly. Together with a A/D conversion at 14 bit, the system ensures accurate and precise data.

Besides A/D conversion and amplification, the headstage also houses a 3-channel stimulator- You can freely design the stimulation patterns via the included software and select each of the 1024 stimulation sites of the chip. One big advantage is that you can record instantly after stimulation. Even on the electrode right next to the stimulation site, recording is possible <1 ms after the stimulation pulse.

Technologically advanced interface board

The interface board receives data from the headstage via iX industrial type B cable. In the interface board you find various analog and digital in- and outputs for synchronization with other instruments. The interface board connects to the computer via USB 3.0 SuperSpeed. The IFB-C is a new interface board generation which is able to receive data fromMEA2100-Headstages, Multiwell-MEA-Headstage, CMOS-MEA5000-Headstage, ME2100-Systems or W2100-Systems. This makes cost-effective combinations with only one interface board and multiple recording systems possible.

IFB-C

Versatile and high performance control software

The CMOS-MEA5000-System is controlled and its data recorded by the software package CMOS-MEA-Control.

The software gives an online, real-time activity overview on the complete chip. You can then define regions of interest and zoom into the areas, where you see most activity. You can also switch off areas to decrease file size.

The software also controls the integrated stimulator. You can freely define 3 independent stimulus patterns, using and adjusting the drag’n’drop modules.

Support and Sales team: Always there for you

Multi Channel Systems has a team of highly-qualified scientists, whose only task is to help you with any issue you might have. No matter whether you want to know whether your biological sample is suitable for MEA recordings or if you have a specific software question: Our support team is here to help you.

We have scientists who earned their PhDs in the fields of biology, biophysics, and biochemistry and specialized in:

Neuronal electrophysiology
Cardiac electrophysiology
Xenopus oocyte research
Patch Clamp

Our sales team has years of experience in electrophysiology, too. They can help you in choosing the right system for your specific application, give you advice on the general set-up and useful accessories. They are happy to visit you in your lab anytime to:

Make a demonstration of our systems
Help you with the installation of a new system
Give you training on hard- and software
Discuss any issues you might have with your application

Worldwide, we are supported by our distribution partners. We have long-lasting cooperations with all of our partners and they are regularly trained on our equipment. Be sure that they have the expertise to advise you on all MCS-Systems.

Please do not hesitate to contact us. Normally, we reply to you within 24 hours. You can contact us via:

Phone +49 7121 90925 25
Email support [at] multichannelsystems.com (Support Team); sales [at] multichannelsystems.com (Sales Team)
Contact form

Benefits

The advantages of the CMOS-MEA5000-System are:

4225 recording electrodes on an active chip
1024 dedicated stimulation sites
25 kHz sampling rate on all channels
Outstanding signal quality
Recordings at sub-cellular level
Powerful software package CMOS-MEA-Control included (free updates available)

Media

Videos:

CMOS MEA5000 System Webinar Recording 10am

Video tutorials:

CMOS-MEA-Control 01 - Overview

CMOS-MEA-Control 02 - First functional tests, test model probe

CMOS-MEA-Control 03 - First functional tests, CMOS array

CMOS-MEA-Control 04 - Main menu

CMOS-MEA-Control 05 - Data source and recorder

CMOS-MEA-Control 06 - Load, sensor current, log

CMOS-MEA-Control 07 - Sensor array tool, activity, ROI

CMOS-MEA-Control 08 - Stimulation

CMOS-MEA-Control 09 - Auxiliary channels, digital port events

Products

Documents

Manual:

MCS_CMOS-MEA5000-System_Quickstart_Manual.pdf

MCS_CMOS-MEA5000-System_Manual.pdf

Data sheet:

MCS_CMOS-MEA5000-System_Datasheet.pdf

Brochure:

CMOS-MEA5000-System_Brochure.pdf

Neuro-Applications-InVitro_Brochure.pdf

Customer voices:

MCS_Customer-Voices_CMOS-MEA5000_Guenther-Zeck.pdf

MEAs

CMOS-MEAs

CMOS-MEA chips for the CMOS-MEA5000-System:

Active CMOS chips with 4225 recording electrodes and 1024 stimulation sites. Electrode grid of 65x65 and spacing between recording electrodes with 16 or 32 µm. Optional with culture chamber or basic/advanced slice chamber.

Accessories

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Products

Publications

Please note: Currently, our publications library includes articles up to 2021 only. Contact us for publications from 2022 – present. Contact Us

Höfling L, Berens P, Zeck G (2019) Probing and predicting ganglion cell responses to smooth electrical stimulation in healthy and blind mouse retina. bioRxiv:609826.

Wickham J, Corna A, Schwarz N, Uysal B, Layer N, Wuttke TV, Koch H, Zeck G (2019) Human cerebrospinal fluid induces neuronal excitability changes in resected human neocortical and hippocampal brain slices. bioRxiv:730036.

Yger P, Spampinato GL, Esposito E, Lefebvre B, Deny S, Gardella C, Stimberg M, Jetter F, Zeck G, Picaud S, Duebel J, Marre O (2018) A spike sorting toolbox for up to thousands of electrodes validated with ground truth recordings in vitro and in vivo. Elife 7.

Bertotti G, Jetter F, Keil S, Dodel N, Schreiter M, Wolansky D, Boucsein C, Boven KH, Zeck G (2017) Optical Stimulation Effects on TiO2 Sensor Dielectric Used in Capacitively-Coupled High-Density CMOS Microelectrode Array. IEEE Electron Device Letters 38.

Zeck G, Jetter F, Channappa L, Bertotti G, Thewes R (2017) Electrical Imaging: Investigating Cellular Function at High Resolution. Advanced Biosystems:1700107-n/a.

Channappa L (2016) Electrical Imaging of Aberrant Activity in Neural Tissues Using High Density Microelectrode Arrays Medizinischen Fakultät.

Leibig C, Wachtler T, Zeck G (2016) Unsupervised neural spike sorting for high-density microelectrode arrays with convolutive independent component analysis. Journal of Neuroscience Methods 271:1-13.

Yger P, Spampinato GLB, Esposito E, Lefebvre B, Deny S, Gardella C, Stimberg M, Jetter F, Zeck G, Picaud S, Duebel J, Marre O (2016) Fast and accurate spike sorting in vitro and in vivo for up to thousands of electrodes. bioRxiv.

Bertotti G, Velychko D, Dodel N, Keil S, Wolansky D, Tillak B, Schreiter M, Grall A, Jesinger P, Möller A, Boven KH, Röhler S, Eickenscheidt M, Stett A, Zeck G, Thewes R (2014) A CMOS-Based Sensor Array for In-Vitro Neural Tissue Interfacing with 4225 Recording Sites and 1024 Stimulation Sites. In: BioCAS. Lausanne.

Eickenscheidt M, Zeck G (2014) Action potentials in retinal ganglion cells are initiated at the site of maximal curvature of the extracellular potential. J Neural Eng 11:036006.

Stutzki H, Leibig C, Andreadaki A, Fischer D, Zeck G (2014) Inflammatory stimulation preserves physiological properties of retinal ganglion cells after optic nerve injury. Front Cell Neurosci 8:38.

Eickenscheidt M, Jenkner M, Thewes R, Fromherz P, Zeck G (2012) Electrical stimulation of retinal neurons in epiretinal and subretinal configuration using a multicapacitor array. J Neurophysiol 107:2742-2755.

Zeck G, Lambacher A, Fromherz P (2011) Axonal transmission in the retina introduces a small dispersion of relative timing in the ganglion cell population response. PLoS ONE 6:e20810.

www.multichannelsystems.com

Innovations in Electrophysiology

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IFB-C

Key features:

The chip is based on complementary metal oxide semiconductor (CMOS) technology, facilitating fast, high-resolution imaging of electrical activity.

CMOS MEA5000 System Webinar Recording 10am

CMOS MEA5000 System Webinar Recording 10am

CMOS-MEA-Control 01 - Overview

CMOS-MEA-Control 01 - Overview

CMOS-MEA-Control 02 - First functional tests, test model probe

CMOS-MEA-Control 02 - First functional tests, test model probe

CMOS-MEA-Control 03 - First functional tests, CMOS array

CMOS-MEA-Control 03 - First functional tests, CMOS array

CMOS-MEA-Control 04 - Main menu

CMOS-MEA-Control 04 - Main menu

CMOS-MEA-Control 05 - Data source and recorder

CMOS-MEA-Control 05 - Data source and recorder

CMOS-MEA-Control 06 - Load, sensor current, log

CMOS-MEA-Control 06 - Load, sensor current, log

CMOS-MEA-Control 07 - Sensor array tool, activity, ROI

CMOS-MEA-Control 07 - Sensor array tool, activity, ROI

CMOS-MEA-Control 08 - Stimulation

CMOS-MEA-Control 08 - Stimulation

CMOS-MEA-Control 09 - Auxiliary channels, digital port events

CMOS-MEA-Control 09 - Auxiliary channels, digital port events

CMOS-MEA5000-System

CMOS-MEA5000+MEA2100-256-System

Multiwell+CMOS-MEA5000-System

CMOS-MEAs

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CMOS-MEA5000-HS

Perfusion-Kit

IFB-C

Test-CMOS-MEA

CMOS-TH - CMOS tissue holder

CMOS dark chamber (CMOS-DC)

C-BNC-Lemo1m

linkedin pixel

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CMOS-MEA5000-System

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IFB-C

Key features:

The chip is based on complementary metal oxide semiconductor (CMOS) technology, facilitating fast, high-resolution imaging of electrical activity.

CMOS MEA5000 System Webinar Recording 10am

CMOS-MEA-Control 01 - Overview

CMOS-MEA-Control 02 - First functional tests, test model probe

CMOS-MEA-Control 03 - First functional tests, CMOS array

CMOS-MEA-Control 04 - Main menu

CMOS-MEA-Control 05 - Data source and recorder

CMOS-MEA-Control 06 - Load, sensor current, log

CMOS-MEA-Control 07 - Sensor array tool, activity, ROI

CMOS-MEA-Control 08 - Stimulation

CMOS-MEA-Control 09 - Auxiliary channels, digital port events

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